Course code 05 36 0095 01
ECTS credits 2
Course title in the language of instruction 
Advanced Biotechnology
Course title in Polish Advanced Biotechnology (Biotechnologia zaawansowana)
Course title in English 
Advanced Biotechnology
Language of instruction English
Course level second-cycle programme
Course coordinator dr inż. Jacek Polak
Course instructors
Delivery methods and course duration
Lecture Tutorials Laboratory Project Seminar Other Total of teaching hours during semester
Contact hours 30 0 30
E-learning No No No No No No
Assessment criteria (weightage) 1.00 0.00
Course objective
  1. Acquainting with biorefineries, techniques of pretreatment and hydrolysis of plant biomass as well as detoxification of the hydrolysates.
  2. Acquainting with selected products of biotechnologies (bioplastics, polyketides, biosurfactants).
  3. Acquainting with biotechnologies used in natural environment protection.
  4. Acquainting with the phenomenon of cometabolism.
  5. Acquainting with bioleaching.
  6. Acquainting with properties of metylotrophic bacteria and methane producing microorganisms as well as their use in biotechnology.
  7. To acquaint students with methods and techniques used during the isolation and purification of bioproducts.
Learning outcomes 
  1. Explain the function of biorefineries.
  2. Describe methods of pretreatment and hydrolysis of plant biomass and detoxification of the hydrolysates.
  3. Describe fundamentals of production and properties of selected products of biotechnology (bioplastics, polyketides, biosurfactants).
  4. Describe biotechnologies used in natural environment protection.
  5. Explain the phenomenon of cometabolism.
  6. Describe methods used in bioleaching.
  7. Characterize methylotrophic bacteria and methane producing microorganisms and describe their applications in biotechnology.
  8. Describe the successive stages of the isolation and purification bioproducts,
  9. Select appropriate bed in chromatographic techniques.
  10. Monitor selected parameters during the purification.
  11. Verify the purity of the purified protein.
  12.  12.	Design efficient purification of bioproducts.
Assessment methods 
All learning outcomes of the course will be verified by the written test.
Prerequisites 
A basic knowledge of general microbiology, biochemistry and biotechnology.
Course content with delivery methods 
Biorefineries ? types, function and products. Pretreatment and hydrolysis of plant biomass and detoxification of the hydrolysates. Selected products of biotechnology (bioplastics, polyketides, biosurfactants). Biotechnology in natural environment protection (bioremediation of polluted soil, water and air). Cometabolism ? definition, examples and application in natural environment protection. Bioleaching ? techniques, importance of extremophilic microorganisms. Characterization of metylotrophic bacteria and methane producing microorganisms and their applications in biotechnology.
Stages and specificity of downstream processing in biotechnology. Single-use systems in bioproduction. Separation of biomass (flocullation and coagulation, filtration, sedimentation, centrifugation). Release of intracellular products (mechanical and non mechanical cell disruption). Inclusion bodies, prevention of their formation and solubilization. Concentration of products (membrane processes, extraction, precipitation). Purification of products (electrodialysis, gel filtration, ion exchange chromatography, hydroxyapatite chromatography, affinity chromatography, hydrophobic interaction chromatography). Cloning vectors for easier purification of target product. Granulation, lyophilization and drying of bioproducts. Examples of purification procedures. Control of purification process.
Basic reference materials 
  1.  Microbial Biotechnology-Principles and Applications, Ed Y.K. Lee, World Scientific Press 2006.
  2.  Advances in Fungal Biotechnology for Industry, Agriculture and Medicine, Ed J.S. Tkacz, L. Lange , Springer 2004.
  3.  Trends in Biotechnology Ed EC Hearns 2006.
  4.  Basic Biotechnology. Third Edition. Ed.: Ratledge C., Kristiansen B. Cambridge University Press, 2006.
  5.  Walsh G., Proteins. Biochemistry and Biotechnology. John Wiley & Sons, Ltd., 2002, 2014.
  6.  Doran P.M., Bioprocess Engineering. Academic Press, 1995.
Other reference materials 
  1.  Podstawy Biotechnologii Przemysłowej, W. Bednarski, J. Fiedurek W WN-T W-wa 2007. Biotechnology 5th ed. J.E. Smith Cambridge University Press 2009.
Average student workload outside classroom 
21
Comments
Last update 2019-10-02 07:49:12